Abstract
Calcium efflux has been studied in barnacle muscle fibres under internal dialysis conditions. Prolonged dialysis of these fibres, with a medium free of ATP and containing 2 mM cyanide and 1 mM iodoacetate, causes the ATP in the perfusion effluent to fall to less than 20 μM. The mean calcium efflux from fibres dialyzed with EGTA buffered solution containing 0.3 μM ionized Ca and no ATP is 0.6 pmol · cm −2 · s −1 . A two-fold stimulation of the calcium efflux is observed when ATP is added to fibres previously dialyzed with an ATP-free medium. Withdrawal of Na + and Ca 2+ from the external medium causes a marked drop in the Ca 2+ efflux in the presence of internal ATP.
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