Abstract

Abstract The effect of ascorbic acid on collagen and acid mucopolysaccharide synthesis and on cell growth has been studied with the use of monalayer tissue cultures of fibroblasts obtained at the time of operation. The addition of ascorbic acid in quantities as low as 50 μg per milliliter of a nutrient medium which consists of 83 per cent Eagle's basal medium, 15 per cent heat deactivated human serum, and 2 per cent embryonic extract 50 , and which is supplemented with antibiotics affected the collagen metabolism of the cells dramatically. In this system, human dermal fabroblasts were capable of synthesizing collagen as long as 3 months after explantation, whereas without supplementation of vitamin C no appreciable collagen synthesis could be observed. Ascorbic acid additions generally enhanced cell growth, whereas they did not show a consistent effect on acid mucopolysaccharide production. Hyaluron c acid rather than sulfated acid mucopolysaccharides ivas synthesized in all the groups whether or not ascorbic acid was added. Possible mechanisms of collagen synthesis are discussed.

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