THE EFFECT OF ARGON ON THE YEAST UNDER CAVITATION

Abstract

Introduction. The process of water purification from contaminants, in particular microbiological, is still relevant. One of the promising and effective physical methods of water disinfection is the use of cavitation, which is characterized by numerous positive results in water purification technology.Aim. To investigate the effect of cavitation and inert argon on the viability of Saccharomyces cerevisiae in water. To determine the effectiveness of argon during cavitation treatment of the water system.Methods. Ultrasonic generator UZDN-2T was used for cavitation treatment of yeast-containing water at frequency of 22 kHz, power 35 W, T = 298 ± 1 K, P = 0.1 MPa, the total duration of the process was 2 hours. A suspension of yeast cells in sterile water taken from a natural water body was used for research. Inert gas (argon) was bubbled into the water throughout the process with simultaneous action of cavitation. The results of calculating the number of the cells before and after the experiments are expressed in colony-forming units.The main results of the study. Treatment of water with high content of yeast with simultaneous action of inert gas and ultrasonic cavitation was carried out. The studied microobjects were Saccharomyces cerevisiae yeast with an initial content of 2.07×104 cells per unit volume of water. It is shown that after 30 min of Ar/US action the number of the microorganisms decreased 2.6 times relative to its initial number. The efficiency of the combined action of argon and cavitation was 99.5% (NMend = 100 CFU/cm3) in the end of the process. Conclusions. Cavitation action in an argon atmosphere on the water purification from yeast was shown. The proportions of dead cells which allowed to describe the process of water disinfection were calculated and active water purification under conditions of argon supply with high yeast content in 1 cm3 of aqueous medium was noted. Almost complete purification of water after two hours of treatment was achieved.Key words: water; cleansing; drіzhі; argon; cavitation