The Effect of Apis mellifera Propolis on RUNX-2 and ALP during Remodeling of Orthodontic Tooth movement

Abstract

Herbal medicine is interesting including treatments that use propolis. Propolis collected by Apis mellifera bees has a very useful composition whose active ingredients are flavonoids, CAPE and saponin. This research aims to analyze the effect of Apis mellifera propolis on RUNX-2 and ALP during the remodeling period caused by the orthodontic tooth movement in Cavia cobaya. It constitutes an experimental laboratory study and a random sampling method. The method used experimental laboratory research on 28 Cavia cobaya, which were divided into four groups consisting of two control groups and two treatment groups which have been given 3% and 5% propolis extract respectively. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Materials and Methods: Propolis is a resin substance produced by bees. Cavia cobaya with orthodontic tooth movement by separator rubber. The control group (Group 1) included clinically healthy Cavia cobaya (n=7), (Group 2) included Cavia cobaya with orthodontic tooth movement (n=7). A treatment group (Group P1) Cavia cobaya have been treated with orthodontic tooth movement and propolis 3%, (Group P2) Cavia cobaya have been treated with orthodontic tooth movement and propolis 5% and observed for 17 days. Samples of maxilla were taken from the subjects for analysis on day 17. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Results: showed that RUNX-2 and ALP expression were found to increase in the tension side. Conclusion: Propolis extract at 5% concentration had ability in bone remodeling by increasing RUNX-2 and ALP expression in the tension area during orthodontic tooth movement.