The Effect of Antiseptics and the Moist Wound Environment on Ulcer Healing: An Experimental and Biochemical Study

Abstract

The effect of topical aqueous chlorhexidine (0.5% and 5%) and Eusol has been studied on the healing of 2 cm skin defects in rats, with measurements of DNA and collagen synthesis as biochemical markers. Healing was also compared under an occlusive dressing (Comfeel Ulcer Dressing, Coloplast) and a non-occlusive dressing (Jelonet, Smith and Nephew). Healing was significantly delayed under Jelonet dressings compared with the Comfeel groups. The hydroxyproline levels, 4 d after wounding were: chlorhexidine 5% 1.82 μg mg−1 (1.46–11.57), Eusol 1.78 (1.13–3.52), Jelonet 2.04 (1.89–3.91), Comfeel 3.21 (1.99–3.46), Comfeel–saline 3.09 (1.99–3.46), chlorhexidine 5% 3.61 (1.47–6.28). The moist wound environment provided by the Comfeel dressings was the most conducive to healing in this study. Chlorhexidine, at a recommended strength of 0.5% (or less), did not impair healing and can safely be used if an antimicrobial is required.