Abstract
Rabbit antiserum prepared against an ATPase-containing tryptic fragment of dynein by Ogawa and Mohri (J. Biol. Chem. 250: 6476-6483) specifically inhibited the ATPase activity of dynein 1 and not that of dynein 2. Varying amounts of this antidynein 1 serum were added to demembranated sperm while they were swimming in reactivating solution containing 1 mM ATP. The sperm continued to form regularly propagated flagellar bending waves, but the beat frequency decreased gradually with time, the greater part of the change occurring in the first 15 min. The beat frequency after 1 h was a function of the amount of antiserum used, and could be as low as 1 Hz. The waveforms of the treated sperm resembled those of normal reactivated sperm except that the bend angles of both the principal and reverse bends were larger in the proximal portion of flagellum. The ATPase activity and corresponding beat frequency of sperm which had been pretreated with varying amounts of antidynein 1 serum for 15 min at 0 degrees C and then diluted were both decreased as a function of the amount of antiserum added, the ATPase activity of homogenized, nonmotile sperm also decreased upon pretreatment with antiserum, but the percentage decrease was less than for motile sperm. For moderate to low concentrations of antiserum, the rates of reaction with motile and with rigor sperm were almost identical. The overall results suggest that antidynein 1 inhibits the functioning of the dynein arms, probably by blocking the ATPase sites of the dynein 1.
Highlights
Rabbit antiserum prepared against an ATPase-containing tryptic fragment of dynein by Ogawa and Mohri
In an attempt to learn more about the involvement of dynein in the mechanism of movement, we have investigated the effects of an antiserum which is known to inhibit dynein ATPase activity in vitro on the motility, waveform, beat frequency, and ATPase activity of demembranated sea urchin sperm
The effect of antidynein 1 serum on reactivated sperm motility is similar to that obtained by extracting the sperm with 0.5 M KCl [7], that is, it markedly reduces the beat frequency while only f l slightly modifying the waveform (Fig. 2)
Summary
Rabbit antiserum prepared against an ATPase-containing tryptic fragment of dynein by Ogawa and Mohri 250:6476-6483) inhibited the ATPase activity of dynein 1 and not that of dynein 2 Varying amounts of this antidynein 1 serum were added to demembranated sperm while they were swimming in reactivating solution containing 1 mM ATP. The ATPase activity and corresponding beat frequency of sperm which had been pretreated with varying amounts of antidynein 1 serum for 15 min at 0~ and diluted were both decreased as a function of the amount of antiserum added, the ATPase activity decreasing more steeply than the frequency. In an attempt to learn more about the involvement of dynein in the mechanism of movement, we have investigated the effects of an antiserum which is known to inhibit dynein ATPase activity in vitro on the motility, waveform, beat frequency, and ATPase activity of demembranated sea urchin sperm. We shall refer to this antiserum as antidynein 1 serum
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
