The effect of anticoagulant on the feeding rate, mortality rate, and infection rate of Aedes aegypti (diptera:culicidae) orally infected with dengue virus-3

Abstract

Blood feeding to female mosquitoes is an important activity for colonization and maintenance of mosquitoes. With increasing awareness of animal welfare and research ethics, mosquito feeding techniques have been developed artificially known as artificial membrane feeding to infect mosquito with Dengue virus per oral. This study aimed to analyze the effect of EDTA and Heparin on the feeding rate.. Two hundred female Ae. aegypti mosquitoes (7–9 days old) were transferred into 8 paper cups covered with net and were starved for at least 24 h before exposure to AMF. The mosquitoes in the paper cups were divided randomly into 2 groups. The first group was administered with EDTA blood containing DENV-3 virus suspension, whereas the 2nd group was administered with heparin blood containing DENV-3 suspension through AMF. The feeding rate of the mosquito was determined 6 h after exposure. The mortality rate was determined at the incubation period of 6 days and 12 days both in EDTA and Heparin Groups. The presence of dengue virus on head squash of mosquito was performed based on immunocytochemical. Feeding rate of Ae. aegypti± in EDTA and Heparin Groups were 45±3.82 %, and 31±3.82% respectively (P= 0.02≤ 0.05). The mortality rates of Ae. aegypti in EDTA and Heparin Groups were 22 %, and 20% respectively at the 6th incubation period (P= 0.382≥ 0.05), whereas the median mortality rate at the 12th incubation period were 22% and 24% respectively (P= 0.278 ≥0.05). The infection rates of Ae. aegypti in EDTA Groups and Heparin Groups were 29.32% %, and 14.23% % respectively at the 12th incubation period (P= 0.068≤ 0.07). This study confirmed that the mean feeding rate, and median infection rate of EDTA Groups were significantly higher than Heparin Groups, but the median mortality rate were not significantly different.