Abstract
Atomic force microscopy (AFM) and fluorescence microscopy was applied to determine the influence of the anti-aging peptides on the morphology and the mechanical properties of keratinocytes. Immortalized human keratinocytes (HaCaT) were treated with two anti-aging bioactive peptides: Acetyl Tetrapeptide-2 and Acetyl Hexapeptide-50 (Lipotec). The AFM measurement of the keratinocyte stiffness were carried after 48 h exposure at an indentation depth of 200 nm. AFM analysis showed increase of the cell stiffness for cells treated with Acetyl Tetrapeptide-2 (P1) in concentration range. Acetyl Hexapeptide-50 (P2) at concentration of 0.05 µg/ml also increased the stiffness of HaCaT cells but at higher concentrations 0.5 and 5 µg/ml cell stiffness was lower as compared to untreated control. Fluorescence microscopy revealed remodeling of actin filaments dependent on the concentration of P2 peptide. The mechanical response of HaCaT cells treated with P2 peptide corresponds to change of transcription level of ACTN1 and SOD2 which activity was expected to be modulated by P2 treatment.
Highlights
In response to still growing demands, the researchers working in pharmaceutical and cosmeceutical fields are developing new, more efficient, bioactive and biocompatible antiaging compounds
First group - signal peptides generally stimulate protein production including the components of extracellular matrix (ECM) like collagen, elastin, glycosaminoglycans and fibronectin
We investigated the influence of two anti-wrinkle bioactive peptides on mechanical properties of HaCaT keratinocytes
Summary
In response to still growing demands, the researchers working in pharmaceutical and cosmeceutical fields are developing new, more efficient, bioactive and biocompatible antiaging compounds. First group - signal peptides generally stimulate protein production including the components of extracellular matrix (ECM) like collagen, elastin, glycosaminoglycans and fibronectin. Signal peptides stimulating activity of fibroblasts resulting in ECM collagen production result in firmer and younger look of the skin. Mechanical properties of the skin change with aging and under influence of various external factors like UV irradiation and environmental chemical pollution (Sobiepanek et al 2016). This leads to a disturbance in synthesis of collagen and other structural ECM and cellular proteins causing the loss of skin firmness and elasticity. We investigated the influence of two anti-wrinkle bioactive peptides on mechanical properties of HaCaT keratinocytes. The sample absorbance was determined at 595 nm wavelengths in a microplate reader (Synergy H4, Biotek) The results are expressed as a percent of untreated control and are an average of three experiments
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Peptide Research and Therapeutics
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
