The effect of anesthesia and surgery on heart rate variability in dogs

Abstract

Heart rate variability (HRV) may be useful for objective assessment of stress and pain in animals. The objective of this study was to describe the effect of anesthesia and surgery on HRV in dogs. We hypothesized that surgery would decrease HRV to a greater degree and for a longer duration than anesthesia alone. Four healthy male dogs (29 ± 2 kg) were instrumented for ambulatory ECG monitoring. Continuous ECG data was obtained for 1 day prior to, and 6 days following anesthesia alone (ANES) or anesthesia plus unilateral stifle arthrotomy (ANSX). The anesthetic protocol included xylazine (0.5 mg kg–1 IM), morphine (0.5 mg kg–1 IM), atropine (0.04 mg kg–1 IM), thiopental (10 mg kg–1 IV) and isoflurane in oxygen. A single dose of morphine (0.5 mg kg–1 IM) was administered at extubation. Time domain analysis of HRV was performed on 5 minutes epochs of artefact- and arrhythmia-free ECG data obtained at 12 noon and 12 midnight on each of the seven experimental days. Mean RR interval, standard deviation of normal R-R intervals (SDNN), and the standard deviation of successive differences in RR intervals (SDSD) were compared to baseline for ANES and ANSX. Pain scores obtained during the day were also evaluated. Significance was set at p < 0.01. There were no differences between groups for any baseline data. Mean RR interval did not differ from baseline on days 1–6 in ANES or ANSX. SDNN and SDSD values at noon were not different from baseline on days 1–6 in ANES or ANSX. At midnight on days 1 and 2, SDNN was significantly decreased from baseline in ANSX, and on day 1 a significant difference between groups existed. ANSX values of SDSD at midnight were significantly decreased from baseline and ANES on day 1. Pain scores for ANSX were significantly greater than baseline on days 1–3, and different from ANES on days 1–5. These results suggest that HRV is decreased following anesthesia plus surgery, and that changes in HRV may be associated with pain.