The effect of Andromed® and coconut water + 20% egg yolk as diluent on semen motility of Belgian Blue cattle

Abstract

The purpose of this study was to observed the potential use of coconut water + 20% egg yolk (CW) as an alternative diluent to replace Andromed®, on semen motility of Belgian Blue (BB) cattle. The sample were fresh semen collected from 3 years old BB using artificial vagina. The fresh semen was diluted into Andromed® diluent (P1) and coconut water + 20% egg yolk (P2). The semen motility (%) was observed at 0, 2, 4, 6, 12, 24 and 48 hours after dilution and then compared between group using t-test. The result showed that Andromed® found to give better motility compared to CW in all observation time. The average of semen motility (%) in accordance with observation time in group P1 was 77,67±2,51, 74,67±2,51, 72,33±2,51, 70,67±1,54, 69,33±1,54, 66,33±2,88 and 63,33±2,88%, respectively. Meanwhile, in P2 was 71,67±2,89, 65,00±5,00, 60,00±5,00, 58,33±2,89, 46,67±7,64, 41,67±7,64 and 26,67±7,64 % respectively. In conclusion, coconut water + 20% egg yolk could be replace the function of Andromed as diluent for AI using liquid semen with maximum storage time no more than 24 hours.