The effect of amylase and cellulase enzymes on biogas production from rice husk waste using solid-state anaerobic digestion (SS-AD) method

Abstract

Biogas comes from an anaerobic biological decomposition process. Biogas is an alternative energy that potential to be developed in Indonesia. One of the various raw materials that can be used in biogas production is rice husk waste. This study analyzes the effect of pretreatment using amylase and cellulase enzymes on the production of biogas from rice husk waste in the Solid-State Anaerobic Digestion (SS-AD) method. Rice husk waste was used as a substrate with a C/N ratio of 25%, and the total solids used was 21%. Rice husk is biomass that is difficult to degrade because it has high lignin content; therefore, pretreatment is necessary. One of them is biological pretreatment using enzymes. The enzymes used are amylase and cellulase enzymes with various concentrations of 9%, 12%, 15%, and 18%. The biogas produced was then measured every two days for 40 days of the study with the volume of biogas as the observed parameter. The results showed that pretreatment with the addition of amylase and cellulase enzymes could increase biogas production. The highest biogas production was obtained by pretreatment of 18% amylase enzyme, namely 1466 mL, and by pretreatment of 18% cellulase enzyme, namely 1075 mL.