The effect of amphotericin B on the K-channel activity of MDCK cells

Abstract

By using the whole-cell patch technique, it is shown that the total outward current is increased, as a function of time, after the addition of amphotericin B to the bathing solution. The whole-cell current is shown to be primarily a K-channel current by the blockage of this current upon application of TEA to the bathing solution. Single K-channel studies, using the outside-out patch-clamp technique, reveal that the single K-channel opening probability increases by a factor of six after the addition of amphotericin B. In addition, single K-channel voltage dependent studies, using the inside-out patch-clamp technique, demonstrate that this increase in opening probability is due to an increase in the amplitude of P o (v) . In contrast to the present belief that amphotericin B simply creates pores in a cell's membrane, these results suggest that amphotericin B can also influence the function of the cell's K-channel proteins.