Abstract
In Osmunda regalis and other fern species a rapid and extensive fall in the level of glutamine synthetase activity occurred when gametophytes were placed in darkness or when treated with ammonia (2 mM). In Osmunda, glutamine synthetase activity was restored in vivo upon re-illumination and in vitro when extracts were incubated with magnesium, glutamate, dithiothreitol and ATP. Light/dark transitions and ammonia had little effect on the levels of nitrogen-assimilating enzymes tested.
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