The effect of aloes burn cream on expression of transforming growth feactor-β1 and epidermal growth factor-like domain 7 in rats deep partial burn wound

Abstract

Objective To investigate the effect of aloes burn cream on expression of transforming growth factor-β1 (TGF-β1) and epidermal growth factor-like domain 7 (EGFL7) in rats deep partial burn wound. Methods Ninety-six male c57bl/6 rats were randomized into four groups (24 rats in each group), each of which was given one of burn wound with 2 cm in diameter on the back. After Saline solution, SD-Ag, blank bases, aloes burn cream applied on each wound, respectively. Tissue wound healing were observed at days 7, 10 and 14 by hematoxylin and eosin (HE) staining; TGF-β1 and EGFL7 level in wound tissues were detected at days 3, 7, 10 and 14 by enzyme-linked immuno sorbent assay (ELISA). Results Aloes burn cream can effectively restore the epithelization and promote the angiogenesis of burn wound; At days 3 and 7, TGF-β1 level in wound tissue of aloes burn cream group reach to (22.72±1.05), (31.58±3.91) μg/g and EGFL7 level reach to (99.67±3.33), (161.60±12.74) μg/g, which are significantly higher than that in control group, respectively (P<0.05). At days 10 post burn, EGFL7 level in wound tissue of aloes burn cream group maintain at (148.25±15.33) μg/g, which is significantly higher than that in SD-Ag gand blank bases groups (P<0.05). Conclusion Aloes burn cream increasing synthesis and release of TGF-β 1and EGFL7 and acceleate wound healing. Key words: Aloes burn cream; Burn; Transforming growth factor-β1; Epidermal growth factor-like domain 7