The effect of allogenic freeze-dried platelet-rich plasma in increasing the number of fibroblasts and neovascularization in wound healing

Abstract

BackgroundTo evaluate the use of allogenic freeze-dry PRP for the full-thickness wound healing process in experimental animals (rabbits). Material and methodsThis study is an experimental research using the posttest-only design, a simple randomized design. Thirty rabbits were randomly divided into six groups (three control groups and three treatment groups), and each group consisted of five subjects. Skin-thick cuts were made on the back of each rabbit. Wounds in the control group were treated using a paraffin gauze, and allogenic freeze-dried PRP was given to the treatment group; observations were made on the 3rd, 9th, and 14th days. The t-test on two random samples and Mann–Whitney were used to process data. The results were significant at p < 0.05. ResultsThere were significant differences in the average number of fibroblasts the control groups and the treatment groups on day 3 (p = 0.009), day 9 (p = 0.023), and day 14 (p < 0.001). There were also major differences in the average amount of neovascularization between the control group and the treatment group on day 3 (p = 0.034), day 9 (p < 0.001), and day 14 (p < 0.001). ConclusionsThe application of allogenic freeze-dried PRP was confirmed to be able to increase the number of fibroblasts and neovascularization in the full-thickness wound healing process in rabbits.