Abstract
Abstract Suspensions of human platelets in isotonic, protein-free artificial media contain lactate dehydrogenase, adenosine triphosphatase (ATPase), and adenylate kinase activities in both the platelet suspensions and the platelet-free ambient media. As platelets are repeatedly resuspended, these enzyme activities increase. The addition of 5 mg. per milliliter of albumin to the resuspension media diminished enzyme loss from platelets. Albumin has no effect upon the lactate dehydrogenase, ATPase, or adenylate kinase activities remaining in the ambient media after platelets are removed by centrifugation. Therefore, the decreased enzyme activities in platelet suspensions containing albumin must be due to an interaction between albumin and platelets. We suggest that albumin may provide the platelet with a protective "protein coat" which diminishes the loss of platelet constituents into the surrounding media.
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