The effect of ageing on parenchymal cell populations in adult female mouse submandibular gland

Abstract

The submandibular gland shows an array of responses that accompany ageing, which are usually modest. However, the submandibular acinar-cell mucin shows a substantial decline in total amount per gland. In the submandibular gland, there is also a loss of secretory parenchyma. A number of factors that could influence either parenchymal loss or a change in its cellular composition were examined in three ages of adult female mice. The goal was to see if there are ageing-related cellular changes that might have an effect on mucin production or secretion. The factors examined included DNA, protein, rates of cell division and apoptosis, cell volume and cellular composition of the parenchymal population. The parenchymal cell composition showed significant differences during ageing, with a substantial decrease in the percentage of acinar cells and increases in the percentage of both types of ductal cell components. This decline in the proportion of acinar cells in the parenchyma also reflected an overall reduction in the total number of acinar cells in the gland. Thus, the change in proportions of cells may potentially be a direct cause of the ageing-related decline in the submandibular acinar-cell mucin. The alteration in cellular composition was not attributable to changes in the cell-division indices; however, there was an increased rate of apoptosis for acinar cells that was significantly different between 3 and 28 months. The apoptotic rate doubled for acinar cells but showed no significant change in ductal cells. This selective change in the rate of apoptosis with ageing suggests that it is one of the main reasons for the decline in the proportion of acinar cells in the submandibular gland.