Abstract
Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effect of donor age on their characteristics and regenerative potential of HEASCs in vitro. The HEASCs were isolated from patients of three groups: (1) <20 years (n = 4), (2) >20 years, <45 years (n = 5), and (3) >55 years (n = 4). For each group, the proliferative capacity, colony-forming ability, surface markers, differentiation ability, wound healing function, and secreted protein were contrastively evaluated and quantified for statistical analysis. It was found that HEASCs were successfully isolated and cultured by an explant culture method. The proliferative rates, osteogenic and chondrogenic differentiation potentials, wound healing ability, and the expression of TGF-β1 and fibronectin protein of HEASCs significantly decreased as age increased. However, the expression of CD90 antigen and the adipogenic differentiation showed an age-related increase in HEASCs. As many degenerative diseases increase in prevalence with age, the age-related changes of the HEASCs proliferation potential, differentiation capacity, and wound healing ability should be taken into account whenever they are intended for use in research or cytotherapy.
Highlights
Mesenchymal stem cells (MSCs) are one of the most prominent stem cell types in the translational medicine due to their regenerative potential and paracrine effect for treating different kinds of diseases [1,2,3]
The Human eyelid adiposederived stem cells (HEASCs) successfully outgrew from all donor age eyelid adipose tissues after 6–20 days of explant culture
The expression of MSC markers was observed in HEASCs from donors of various ages by flow cytometry analysis
Summary
Mesenchymal stem cells (MSCs) are one of the most prominent stem cell types in the translational medicine due to their regenerative potential and paracrine effect for treating different kinds of diseases [1,2,3]. Cell therapy with bone marrow-derived stem cells (BMSCs) is under investigation in preclinical studies and clinical trials, the use of adipose-derived stem cells (ASCs) involves less religious and ethical concerns than using bone marrow-derived stem cells because they are obtainable from medical waste [4, 5]. There has been the growing interest in applying ASCs as a potential cell source in stem cell-based therapy and tissue engineering. Human eyelid adiposederived stem cells (HEASCs) are novel MSCs and originated from the neural crest source. It is possible that HEASCs may be an excellent candidate therapeutic adipose-derived stem cell type for treating complicated diseases
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