Abstract
The aim of this study is to investigate the antibacterial properties and characterization of chitosan-silver nanoparticle composite materials. Chitosan-silver nanoparticle composite material was synthesized by adding AgNO3and NaOH solutions to chitosan solution at 95°C. Different concentrations (0,02 M, 0,04 M, and 0,06 M) of AgNO3were used for synthesis. Chitosan-silver nanoparticle composite materials were characterized by Transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet (UV) spectrophotometer, and Fourier transform infrared (FTIR) spectrometer techniques.Escherichia coli,Acinetobacter baumannii,Staphylococcus aureus,Enterococcus faecalis,Pseudomonas aeruginosa, andStreptococcus pneumoniaewere used to test the bactericidal efficiency of synthesized chitosan-Ag nanoparticle composite materials. The biological activity was determined by the minimum bacterial concentration (MBC) of the materials. Antibacterial effect of chitosan-silver nanoparticle materials was increased by increasing Ag amount of the composite materials. The presence of small amount of metal nanoparticles in the composite was enough to significantly enhance antibacterial activity as compared with pure chitosan.
Highlights
Chitosan obtained from a natural polymer chitin has antibacterial feature
X-ray diffraction (XRD) patterns indicated the existence of Ag nanoparticles in chitosan matrix, Fourier transform infrared (FTIR) spectra provided information of structural change, UV spectra gave Ag concentration in chitosan, and Transmission electron microscopy (TEM) images showed that the size of the Ag nanoparticles well penetrated into chitosan
UV absorption peak of chitosan-Ag nanoparticles prepared by other researchers was recorded in the range 410–420 nm [8, 13,14,15,16]
Summary
Chitosan obtained from a natural polymer chitin has antibacterial feature. Chitosan is an environmental friendly material because of its biodegradability. Nontoxic and antibacterial features of chitosan make it usable for many areas related to human health [1,2,3,4,5]. Silver (Ag) ion has been used for a long time as antibacterial agent due to its strong inhibiting effect on bacteria. Nanoparticle Ag has taken considerable attention to provide maximum bactericidal effect with minimum amount of Ag [7,8,9,10]. Chemical reducing agents such as NaBH4, ascorbic acid are used for preparation of Ag nanoparticle [7,8,9,10,11]
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