Abstract
Impaired wound healing is a significant medical problem. Recently, cell-based therapy focused on stem cells has been developed to overcome the challenges of defective wound healing. In this study, we aimed to evaluate the effectiveness of adipose-derived stem cells (ASCs) in promoting wound healing, using different techniques for administering them. Dorsal full-thickness skin defects (1 × 1 cm) were created in three groups of mice that received intravenous ASCs by intravenous injection, intramuscular injection, and topical application, respectively. Three control groups received saline in the same ways. Wound healing was assessed clinically, wounds were examined histologically, and GFP-labelled ASCs were detected with an IVIS imaging system. The results revealed that ASCs accelerated wound healing independent of their mode of administration. Histological examination showed that the ASCs accelerated reepithelialization, and IVIS analysis indicated that many ASCs were present in the wound area and disappeared after wound healing.
Highlights
Impaired wound healing is a significant medical problem with a clinical and socioeconomic cost
Mesenchymal stem cells can be isolated from various sites, including bone marrow, adipose tissue, and cord blood [4,5,6,7]
The Adipose-derived stem cells (ASCs) expressed high levels of the stem cell markers CD73 and CD105 as judged by immunofluorescence staining
Summary
Impaired wound healing is a significant medical problem with a clinical and socioeconomic cost. Recent advances in regenerative medicine have revealed promising approaches to overcoming this probem [1, 2]. These are mostly focused on stem cells, which are specialized self-renewing cells with the ability to differentiate into multiple cell types [3]. Bone marrow-derived stem cells (BMSCs) can facilitate tissue repair by producing growth factors, cytokines, and extracellular matrix [6, 8] and promoting the migration of other cells [9]. Adipose-derived stem cells (ASCs) are multipotent cells in adipose tissue with characteristics similar to those of BMSCs [4, 10]. In contrast to the invasive procedure needed to harvest BMSCs and their low yields, ASCs are easy to harvest and there is minimal donor morbidity. [5, 11] Since the first report by Zuk et al [12, 13], many studies have confirmed that ASCs have the same favorable effects as BMSCs on wound repair, immunomodulation, and antiapoptotic activity [12,13,14,15,16]
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