The effect of adiponectin on the phenotype and function of Jaws II cells

Abstract

Many adaptive immune responses are impaired by obesity. Adiponectin is an anti‐inflammatory adipokine that may play a role in mediating the immunoregulatory effects of obesity. The goal of the present study was to determine if adiponectin alters the phenotype and function of dendritic cells (DCs), potent antigen presentation cells important in activating antigen‐specific T cells. In vitro studies were performed utilizing an immortalized murine dendritic cell line, Jaws II, which responds to cytokine stimulation and presents antigen similarly to bone marrow‐derived DCs. Jaws II cells were evaluated at baseline or stimulated with LPS for 24 hours to induce maturation, and treated with vehicle or two forms of adiponectin (mouse recombinant and globular domain) at 1 and 10 μg/mL for 48 hrs. At baseline, and following LPS stimulation, Jaws II cells were harvested, counted and MHCI, MHCII, and CD80 expression was quantified with and without adiponectin treatment by flow cytometry. IL‐6 production was also quantified in the harvested supernatants. Adiponectin treated Jaws II cells had a significantly greater LPS‐induced increase in MHC class II expression (20–130%) and IL‐6 production (<50%) as compared to vehicle treated control cells. These results suggest that adiponectin alters the phenotype and function of DCs and may play a role in antigen presentation or other important functions of DCs.Grant Funding Source : Penn State internal funding