The effect of adding hyaluronic acid to calcium phosphate on periapical tissue healing following periradicular surgery in dogs

Abstract

ObjectiveThis study aimed to evaluate the effect of adding exogenous hyaluronic acid (HA) to Beta-tricalcium phosphate (CP) on osseous tissue healing of induced apical lesions following periradicular surgery in a dog model. MethodsIn the first and second sessions, periapical lesions were induced by exposing pulp cavities of selected teeth for 7 days then sealing them with glass ionomer for 60 days. Root canals were then cleaned, shaped and obturated. Surgical treatment included buccal osteotomy to expose the root apex and root-end resection and filling. Osteotomy cavities were randomly allocated to two study groups 12 samples each according to the graft materials; CP or combination of HA and CP. The graft materials were prepared and applied in each animal in alternate quadrant in a randomized manner. Animals were sacrificed after 60 days and bone treated cavities were prepared for histological study and histomorphometric analysis for the area percentage of new bone tissue and trabecular bone thickness. ResultsAll samples displayed signs of regeneration as newly formed bone tissue and fibrovascular connective tissue within the treated cavity sites with complete resorption of the implemented materials. The newly formed bone consisted mainly of osteoid bone trabeculae with some more mature dense bone present at the periphery of cavity site. There was no significant difference in the percentage of newly formed bone tissue (P > 0.05) and bone trabeculae thickness (P > 0.05) between the two study groups. ConclusionsAddition of exogenous HA to CP after periradicular surgery did not improve the histological outcome of osseous tissue healing in a dog model.