The effect of adding different levels of date Kernel powder treated with oyster mushroom P.ostreatus in stimulating amylase and protease enzymes in the intestine of Cprinus carpio L.

Abstract

The main objective of this study was to investigate the stimulation of some digestive enzyme being ( amylase and protease ) in the digestive duct of common carp ( Cyprinus carpio ) as result of feeding the experiment fishes with diets treated with different level of date kernel meal treated with oyster mushroom ( P .ostereatus ) . The experiment fishes were collected from local farm, 48 fish were selected with mean weight of 24 ± 3 g / fish , then placed glass aquaria , 6 fishes each aquarium and the experimental carried out in the duplicates run ( 2 aquaria for each group). The different level of date Kernel meal treated with oyster Mushroom were used instead of yellow corn meal ( 33, 66 and 100 % ) to prepare the experimental diet which designated as T1, T2 and T3 respectively , addition to the control diet free of date kernel meal . Experimental fishes were fed randomly for 70 days with the above diet. After that, the fishes fed with 4 % of live weight. The current study included estimating the enzymatic activity in different parts of the gastrointestinal tract during the experiment. The result showed the digestive enzyme were found in all studied parts of digestive duct intestine (anterior, middle and posterior) at after starvation 5 days. The results of the statistical analysis indicated that the values of the posterior part of the intestine differed significantly from those of the anterior and middle sections. The three treatments showed the enzymatic activity enzymes in the anterior and middle part after 70 days of feeding the fish, which differed significantly (P<0.05) with the posterior part of the intestine and for all the treatments. The protease enzyme recorded its highest activity in the anterior and middle part of the intestine in treatment T2 (33% date kernel meal), and well amylase showed its highest activity in the anterior and middle part of the intestine.