Abstract

Simple SummaryIn rabbit farms, artificial insemination is usually accepted using semen preserved around 18 °C. However, the use of cryopreserved rabbit semen is limited, due to excess oxidative stress and produce sperm dysfunction. The advancements in nanotechnology tools have allowed molecular-based targeting of cells through effective, safe, and biocompatible magnetic nanoparticles with promising potentials in reproductive sciences. In these regards, the current work aimed to explore the potential role if the effect of curcumin nanoparticles supplementation in semen extender on post/thawed rabbit sperm quality. Results revealed that the CUNPs (1.5 µg/mL) showed superior enhancements impacts for the post-thawing sperm motion and redox status, as well as a significant reduction in apoptotic and necrotic sperm cells. This confirmed the constructive application of nanoparticle to enhance the cryopreserved rabbit’s sperm function.The cryopreservation process adversely affects sperm function and quality traits, causing some changes at biochemical and structural levels, due to mechanical, thermal, osmotic, and oxidative damage. Supplementation with curcumin nanoparticles could prevent and even revert this effect and could enhance the post/thawed sperm quality in the rabbit. The study amid to explore the effect of curcumin (CU) and curcumin nanoparticles (CUNPs) supplementation in semen extender on post/thawed rabbit sperm quality. Twelve fertile, healthy rabbit bucks were included, and the ejaculates were collected using artificial vaginas. Rabbit pooled semen was cryopreserved in tris-yolk fructose (TYF) extender without any supplement (control group) or extender supplemented with CU at levels of 0.5, 1 or 1.5 µg/mL (CU0.5, CU1.0, and CU1.5, respectively) or CUNPs at levels of 0.5, 1, 1.5 (CUNPs0.5, CUNPs1.0, and CUNPs1.5, respectively) and was packed in straws (0.25 mL) and stored in liquid nitrogen (−196 °C). Results revealed that CUNPs1.5 had a positive influence (p < 0.05) on post-thawing sperm progressive motility, viability, and membrane integrity as compared with the other groups. Percentages of dead sperm, abnormalities, early apoptotic, apoptotic, and necrotic sperm cells reduced (p < 0.05) in CUNPs1.5 as compared to other treatments. Using 1.5 µg/mL of CUNPs significantly improved total antioxidant capacity (TAC), GPx, while MDA and POC reduced (p < 0.05) in CU1.5 in comparison with other groups. SOD values were enhanced (p < 0.05) in CUNPs1.0 and CUNPs1.5 in relation with other treatments. Conclusively, the addition of curcumin and its nanoparticles to the extender can improve the post-thawed quality of rabbit sperm via redox signaling and reduce the apoptosis process.

Highlights

  • Gamete cryopreservation is a critical system for artificial insemination (AI) that permits cheap, the global distribution of gametes with superior genetics

  • In In order to confirm thethe synthesis of curcumin nanoparticles (CUNPs), thethe efficiency testing of of order to confirm synthesis of curcumin nanoparticles (CUNPs), efficiency testing

  • The present study found that the supplementation of nano-curcumin to the freezing medium in rabbit semen helps in alleviating the damage influence on the macromolecules like abnormal nucleus with necrosis chromatin, damage in the plasma membrane and crucial organelles like mitochondria

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Summary

Introduction

Gamete cryopreservation is a critical system for artificial insemination (AI) that permits cheap, the global distribution of gametes with superior genetics. Studies have indicated higher fertility rates and prolificacy were employed with cooled semen for a short time (36 h) [2]. AI with cryopreserved rabbit sperm had inferior prolificacy or fertility than fresh or cooled semen [1]. The excess of OS production throughout the freezing/thawing process modifies protein and lipid structure, reduction viability, and motility; cause loss to mitochondria, acrosomes, tails, and enhance DNA fragmentation in sperm [1,5]. The freezing and thawing procedures prompt rearrangement of lipid membranes, resulting in increased intracellular calcium and fluidity, which initiates precipitation, triggering several instabilities of cell physiology [5,6,7]

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