The effect of adapting cellulose degrading microorganisms to 25°C providing energy sources for biological sulphate removal

Abstract

Vast volumes of Acid Mine Drainage (AMD) are still being generated in South Africa, due to decant from both active and closed mines. Research to find a cost effective, environmentally friendly treatment system to reduce the salinity and to neutralise the acidity of AMD is ongoing. The study presented here showed that high sulphate removal efficiencies were achieved applying the biological treatment technology, thereby using the degradation products of grass-cellulose as the carbon and energy sources. The process was conducted at 25 degrees C, as opposed to 37 degrees C described previously, using a one-stage hybrid reactor system, treating both sulphate rich synthetic feed water and pre-treated AMD. The results showed that the fermentation microbes, originating from rumen fluid, derived from cattle, could generate the carbon and energy sources for the sulphate reducing bacteria at 25 degrees C. When comparing the results obtained at 25 degrees C with those obtained at 37 degrees C, it was observed that these were similar. However, at the higher temperature a faster flow-rate to the reactor was possible. The findings implied that the biological sulphate removal system described can be operated more economically at 25 degrees C than at 37 degrees C, as heating the reactor system can be omitted.