The effect of A375 cells corylin melanin synthesis

Abstract

Objective To study the effect of Corylin on A375 cells melanin synthesis,and explore its mechanism. Methods The cells were randomly divided into the control group, the estradiol group, and corylin group including 10-3 μmol/L, 10-2 μmol/L, 10-1 μmol/L, 1 μmol/L, 10 μmol/L, 100 μmol/L. Estradiol estradiol intervention group were given 10-3 mol/L. Corylin group (10-3 μmol/L, 10-2 μmol/L, 10-1 μmol/L, 1 μmol/L, 10 μmol/L,100 μmol/L) were given 10-3 μmol/L, 10-2 μmol/L, 10-1 μmol/L, 1 μmol/L, 10 μmol/L, 100 μmol/L corylin intervention. The activity of proliferation were detected by MTT, NaOH method, dopa oxidation , both melanin content and tyrosinase activity (tyrosinase, TYR). TYR, yrosinase related protein (tyrosinase related protein, TRP)-1 and TRP-2 expression levels of mRNA were determined by RT-PCR. Results Compared with the control group, 10, 100 μmol/L of Corylin group cell proliferation rate significantly decreased (P<0.01). The 1 μmol/L, 10-1 μmol/L, 10-2 μmol/L of Corylin group cell melanin content, TYR significantly decreased (P<0.01 or P<0.05). The 1 μmol/L corylin group TYR (0.303 ± 0.003 vs. 0.628 ± 0.012), TRP-1 (0.313 ± 0.008 vs. 0.677 ± 0.022), TRP-2 (0.456 ± 0.028 vs. 0.687 ± 0.020) mRNA expression level significantly decrease (P<0.01). Conclusions The results showed that Corylin could inhibit melanin synthesis, which worked probably through inhibiting the activity of TYR and cutting the mRNA expressions of TYR,TRP-1/2. Key words: Psoralea corylifolia; Isoflavones; Melanins; Monophenol monooxygenase