Abstract

A vitamin D-free fat, namely, purified cottonseed oil, incorporated in an adequate-calcium, low-phosphorus, cereal-free rachitogenic ration, when fed to young rats increased the absorption and retention of phosphorus but decreased the percentage of bone ash. This apparently was caused by an increase in growth which made the demand for phosphorus of certain soft tissues dominant over those of bone. When fat was fed with the basal ration supplemented with an adequate amount of phosphorus, the demand of both types of tissue could be met; calcification of bone was increased and the phosphorus content of certain soft tissues such as the liver was also increased. It follows, that data on calcification of bone cannot always be used as a criterion of the over-all state of phosphorus equilibrium in the animal.

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