The effect of a new product based on quercetin and a thick extract of carrot seed root on the histological state of the rat cardiac muscle in the adrenaline-hydrocortisone-induced myocardial infarction

Abstract

Aim. To study the effect of tablets with a thick extract of carrot seed root and quercetin on the histological state of the rat cardiac muscle in the experimental adrenaline-hydrocortisone-induced myocardial infarction. Materials and methods. The cardioprotective effect of tablets with a thick extract of carrot seed root and quercetin was studied on the model of acute adrenaline-hydrocortisone-induced myocardial infarction caused by subcutaneous administration of 0.1 % adrenaline hydrochloride solution in the dose of 1 mg/kg and 2.5 % hydrocortisone acetate emulsion in the dose of 12.5 mg/kg of the animal weight twice daily for a week. The tablets under research in the dose of 200 mg/kg and the reference drugs – “Thiotriazolin” and “Cratal” tablets were administered for 14 days – 7 days before the infarction modeling and 7 days of the myocardial infarction development, after which their effect on the histological state of the rat heart muscle was studied. Results. Modeling of the adrenaline-hydrocortisone-induced myocardial infarction in rats is accompanied by the appearance of focal necrotic changes in muscle cells, which in some cases are accompanied by initial regenerative manifestations in the form of granuloma formation; dystrophic processes of cardiomyocytes. The therapeutic and prophylactic administration of tablets from a thick extract of carrot seed root and quercetin on the model of the adrenaline-hydrocortisone-induced myocardial infarction has the cardioprotective effect, reducing the severity of necrotic and dystrophic changes in the cardiac muscle. Conclusions. As a result of modeling of the adrenaline-hydrocortisone-induced myocardial infarction in rats, focal necrotic changes in muscle cells were observed, sometimes accompanied by the initial formation of a matrix consisting of young fibroblasts; dystrophic processes of cardiomyocytes, in particular myocytolysis, fluctuations in the size of nuclei. The therapeutic and preventive administration of the tablets studied on the model of the adrenaline-hydrocortisone-induced myocardial infarction has the cardioprotective effect, reduces the severity of necrotic and dystrophic changes in the cardiac muscle. By the cardioprotective effect expressiveness, tablets with a thick extract of carrot seed root and quercetin on the model of the adrenaline-hydrocortisone-induced myocardial infarction are not inferior to the reference drugs – “Thiotriazolin” and “Cratal” tablets. The results experimentally substantiate the feasibility of creating a new combined drug from a thick extract of carrot seed root and quercetin for the prevention and pharmacocorrection of a cardiovascular pathology.