Abstract
The effect of a small dose of antigen, injected intradermally as a simple skin test, on the state of cell-mediated immunity in the guinea pig has been examined. Reduced migration inhibition (MIF) of peritoneal exudate cells (PEC) from animals immunized and skin tested 7 days later was shown against specific and nonspecific antigen and exogenous lymphokine. Lymphocyte cultures from skin-tested guinea pigs showed increased [ 3H]thymidine incorporation when unstimulated by antigen or mitogen. As a result stimulation indices appeared decreased. When sera from skin-tested and non-skin-tested animals were assayed for their migration inhibitory potency against normal PEC, sera from skin-tested animals exhibited a MIF like activity. Evidence for mitogenic activity could also be found when normal unstimulated blood lymphocytes were cultured with 45% skin-tested guinea pig serum in the culture medium. No difference in hemagglutinating antibody titers between skin-tested and non-skin-tested animals was found. It is concluded therefore that a simple skin test may affect the in vitro parameters of cell-mediated immunity and this could be related to a systemic release of lymphokines in vivo.
Published Version
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