Abstract
The effect of paracetamol (4-acetamidophenol) on PG synthetase activity was tested in bull (BSVM) and ram (RSVM) seminal vesicle microsomes. In the presence of glutathione (165 μM) and hydroquinone (45 μM) in BSVM paracetamol (up to 67 μM) had no effect or inhibited ( IC 50 = 1500 μ M ) the enzymic activity. In the absence of cofactors paracetamol (67–667 μM) stimulated the generation of PGs in BSVM. Also in RSVM paracetamol (33–333 μM) stimulated PG and malondialdehyde generation as well as oxygen consumption, provided that the exogenous cofactors were omitted from the incubation mixture. Hydroquinone (150 μM) was able to abolish the stimulatory effect of paracetamol on the enzymic activity. We conclude that paracetamol can replace hydroquinone and other cofactors for cyclo-oxygenation of arachidonic acid in BSVM and RSVM. The inhibitory effect of paracetamol on PG synthetase activity occurs only in the abundance of exogenous cofactors in the microsomal preparation.
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