The effect of 400 nm femtosecond laser and Zn(II)chlorin e6 methyl ester (Zn(II)e6Me) photosensitizer on disinfection of radicular dentin bonded to glass fiber post

Abstract

AimTo assess and compare push-out bond strength (PBS) when canal disinfected with 5.25% NaOCl, 400 nm femtosecond laser, and natural PS activated by PDT (CP and Zn (II) Ce6 methyl ester) on bond efficacy of radicular dentin bonded to glass fiber post. Material and methodsA sum of 40 non-traumatic, cautiously extracted with closed apex human mandibular premolars were collected and decoronated up to the cement-o-enamel junction. Canals of all specimens were cleaned and shaped using a Protaper universal NiTi system via a crown-down approach, then finally dried out and obturated with gutta-percha. Post alignment space was formed with peso reamers and sanitized. Then samples were randomly allocated into four groups as per the radicular dentin designated disinfection protocols. Group 1 disinfected with curcumin photosensitizer + EDTA, group 2: 5.25% NaOCl and EDTA, group 3: Zn (II) Ce6 methyl ester and EDTA and group 4 sanitized with 400 nm fs-laser + EDTA. GFP was cemented into the radicular canal space and specimens were placed in a thermocycler. Segmentation of the root was done at three levels (coronal, middle, and apical) and positioned in a universal testing machine for PBS analysis. Failure mode analysis was performed using a stereomicroscope and statistical analysis was executed by one-way ANOVA and Tukey multiple comparison tests at a statistically significant level of p < 0.05. ResultsThe highest PBS was revealed by group 2 canal disinfected with 5.25% NaOCl +17% EDTA (control) at coronal (9.49 ± 1.42 MPa) and middle (7.545 ± 0.15 MPa) root levels. Whereas, the lowest PBS was displayed by group 3: Zn (II) Ce6 methyl ester +17% EDTA at coronal (6.31 ± 0.54 MPa) middle (4.85 ± 0.11 MPa) root levels respectively. Intergroup comparison presented that radicular dentin disinfected with 400 nm femtosecond laser + 17% EDTA substantiated comparable PBS result to group 2 (control) at all three root levels (p > 0.05). Likewise, root surface disinfection with Curcumin activated by PDT+17% EDTA unveiled no statistically significant result for specimens disinfected with Zn(II) Ce6 methyl ester +17% EDTA(p > 0.05). Furthermore, apical root segments in all groups showed a substantial decrease in PBS value and were comparable (p > 0.05). ConclusionThe bond efficacy of glass fiber post to radicular dentin disinfected with 400 nm femtosecond laser has the potential to be used as an alternative to conventional sodium hypochlorite.