Abstract
1. 1. Chemical modification of carboxyl groups on the chloroplast membrane with a water-soluble carbodiimide plus a nucleophile caused inhibition of Ca 2+ binding. 2. 2. Both binding sites were affected and showed a decrease in the number of binding sites and an increase in the dissociation constant. 3. 3. Cation-induced changes in chlorophyll a fluorescence and structural changes ( ΔA 540) were inhibited at the same carbodiimide concentrations as Ca 2+ binding, emphasizing the relationship between these processes. 4. 4. Chloroplasts that were illuminated with high intensity light for short time periods showed a decrease in the carbodiimide-mediated inhibition of Ca 2+ binding.
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