The Effect and the Mechanism of miR-209 Targeted Regulating PI3K (Phosphatidylinositol3-Kinase)/AKT (Protein Kinase B)/FOXO3a (Forkhead box O3) Signaling Pathway on Glioma Stem Cells

Abstract

To explore the role and mechanism of miR-209 target regulating PI3K/Akt/FOXO3a in glioma. GSCs were isolated from the lesions of glioma patients, cultured, passaged and characterized. Set blank control group (with saline solution), miR-209-mimics group (cells transfected with miR-209-mimics) and negative control group (cells transfected with meaningless sequences). After cell transfection, observe the transfection efficiency of miR-209 MIC, detect the miR-209 mRNA expression level and biological peptide ions such as proliferation, migration, invasion, and withering, and detect the expression of PI3K/Akt/FOXO3a-related proteins (PI3K, p-Akt, FOXO3a). The miR-209mRNA expression level in the miR-209 mimics group was much higher (P < 0.01), and they two had indifferent differentiation (P >0.05); 24, 48 and 72 hours after transfection, the cell migration, proliferation rate and invasion ability of the miR-209 mimics group were much stronger (P <0.05) and the cell apoptosis rate at 24, 48, and 72 hours after transfection was much less (P < 0.01) and they two had no scientific differences (P > 0.05). The PI3K and p-Akt protein expression in the glioma stem elements of the miR-209 transfected group was much higher (P <0.01). The expression of FOXO3a was much less (P <0.01), while the standard level of PI3K, P-Akt and FOXO3a protein had no obvious difference (P >0.05). miR-209 can activate PI3K/Akt/FOXO3a to promote the growth, reproduction and invasion of GSCs, and control the cells withering system. This will provide new avenues for clinical trials.