Abstract
Tescalcin (TESC) is an EF-hand calcium binding protein that is differentially expressed in several tissues, however it is not reported that the expression and functional roles of TESC in colorectal cancer. Levels of messenger RNA (mRNA) and protein expression of TESC in colorectal cancer tissues were assessed using RT-PCR, real time PCR, immunohistochemistry, and clinicopathologic analyses. Quantitative analysis of TESC levels in serum specimens was performed using sandwich ELISA. Colorectal cancer cells transfected with TESC small interfering RNA and short hairpin RNA were examined in cell proliferation assays, phospho-MAPK array, and mouse xenograft models. Here we demonstrated that TESC is overexpressed in colorectal cancer (CRC), but was not expressed in normal mucosa and premalignant dysplastic lesions. Furthermore, serum TESC levels were elevated in patients with CRC. Knockdown of TESC inhibited the Akt-dependent NF-κB pathway and decreased cell survival in vitro. Depletion of TESC reduced tumor growth in a CRC xenograft model. Thus, TESC is a potential diagnostic marker and oncotarget in colorectal cancer.
Highlights
Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide
In a search for possible diagnostic markers by cDNA microarray, we identified TESC as a gene that is over-expressed in CRC (Supplementary Table 1)
TESC is a member of the calcineurin homologous protein (CHP) family and, like other family members, possesses an EF-hand calciumbinding domain
Summary
Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. In the United States, CRC is the third most common form of cancer and the third leading cause of cancer-related death in both men and women [1,2]. Aggressive metastatic cancers are characterized by their high capacity for migration and subsequent invasion and adhesion in distant organs [5]. Acquisition of these properties by cancer cells involves specific changes in the expression level of several genes at the transcriptional and translational level
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
