The effect of echinochrome A on endogenous intoxication in white rats with bleomycin-induced pulmonary fibrosis in the early stage of postnatal ontogenesis

Abstract

Echinochrome A (2, 3, 5, 6, 8-penta-hydroxy-7-ethyl-1,4-naphthoquinone) – quinoid pigment of marine invertebrates, with pronounced antioxidant anti-radical properties. In this experiment, Wistar rats were divided into three groups. The fi rst group – «bleomycin» – the administration of bleomycin to rats at the age of 30 days, once, intraperitoneally, at a dose of 1 mg/kg. The second group «bleomycin + echinochrome» – the introduction of bleomycin, as in the fi rst group, as well as the introduction of echinochrome A per os at a dose of 10 mg/kg for 5 days (on the day of bleomycin administration, as well as on 2-, 3-, 4 - and the 5th day after that). The third group – «control». In the lungs and blood of rats with bleomycin-induced pulmonary fi brosis (group «bleomycin»), we observed the development of oxidative stress, an increase in the level of middle weight molecules (MWM), a shift in the ratio of MWM λ-280 / MWM λ-254 in side of the toxic fraction. These metabolic processes characterized endogenous intoxication and were more pronounced at the organ level (in the lungs) than at the system level (in the blood). Introduction of Echinochrome A (group «bleomycin + echinochrome») prevented the development of pneumofi brosis, signifi cantly reduced the metabolic manifestations of endotoxicosis in the lungs and completely eliminated it in the blood of animals.