The early stage of spinal cord injury in rats, promotes the recovery of nerve function by inhibiting the expression of autophagy associated protein

Abstract

Objective To investigate the role of autophagy in the recovery of neurological function at the early stage of spinal cord injury in rats and its mechanism of action. Methods The production and development of autophagy after spinal cord injury in rats and its relationship with spinal cord injury were analyzed by means of Western blotting, Hematoxylin and eosin staining and motor function score of spinal cord injury in rats basso beattie bresnahan (BBB) score. Results According to the motor function score of spinal cord injury in rats: compared with sham group, the autophagy related gene beclin-1, autophagy gene 12 (ATG12), Microtubule-associated protein 1 light 3 (LC3B) mRNA expression was significantly lowered after spinal cord injury in rats (P=0.000, 0.001, 0.000). Autophagy related protein Beclin-1, LC3BⅡ in the early stage of spinal cord injury was significantly lower than in group sham (P=0.001, 0.000). According to the BBB score: spinal cord injury of nerve function was restored within a week, but the application of rapamycin induced autophagy, recovery was significantly affected by the block, and with a higher mortality (P=0.000). Compared with the pathological results, Compared with the Rapamycin group, the 6-amino-3-methyl purine (3-MA) groups recovered significantly after spinal cord injury, and the glial cells proliferated obviously. Conclusion The early stage of spinal cord injury in rats promotes the recovery of nerve function by inhibiting expression of autophagy associated protein, which may be related to the proliferation and survival of glial cells by decrease of the level of autophagy Key words: Spinal cord injury; Autophagy; Rapamycin; Glial cells; 6-amino-3-methyl purine; Microtubule-associated protein 1 light 3