The dysregulation of movement and reproductive capacity in Caenorhabditis elegans exposed to Triclosan du-ring pregnancy

Abstract

Objective To observe the effect of Triclosan(TCS) exposure on Caenorhabditis elegans(c.elegans) F1 generation of locomotory behavior, brood size, and generation time. Methods The trial included a control group and 4 TCS treatment groups with different doses (100 nmol/L, 1 μmol/L, 10 μmol/L, 20 μmol/L), the exposure time being 24 hours, the effect of c. elegans' head thrashes, body bending frequency, the brood size and generation time was observed. Results (1)The control group exposed to 100 nmol/L, 1 μmol/L, 10 μmol/L, 20 μmol/L TCS, their head thrash frequency of c. elegans F1 was(109.40±8.61) times/min, (84.70±7.82) times/min, (76.35±7.44)times/min, (74.74±5.93)times/min, (71.95±4.19)times/min, respectively, the head thrash ability of c. elegans was significantly inhibited(F=62.245, P<0.01). (2) When the control group was exposed to 100 nmol/L, 1 μmol/L, 10 μmol/L, 20 μmol/L TCS, the frequency of c. elegans F1 body bent was (19.94±2.46)times/20 s, (15.13±1.99)times/20 s, (14.63±2.31)times/20 s, (14.69±1.96)times/20 s, (12.00±1.86)times/20 s, respectively, and the comparative differences between groups were statistically significant(F=25.636, P<0.01). (3) When the control group was exposed to 0, 100 nmol/L, 1 μmol/L, 10 μmol/L, 20 μmol/L TCS, the body sizes of the c. elegans F1 generation was (286.83±6.01)articles, (273.33±6.41)articles, (214.17±7.25)articles, (173.67±9.20)articles, (118.50±6.98)articles, respectively, the brood size of the C. elegans F1 generation exposed to 100 nmol/L, 1 μmol/L, 10 μmol/L, 20 μmol/L TCS levels, were reduced by 4.71%, 25.60%, 39.45%, 58.67%, the ge-neration time of the c. elegans' F1 generation was shortened by 2.14%-5.38% in the TCS treatment groups compared with the control group(F=27.520, P<0.01). Conclusions After c. elegans exposure to TCS, locomotory behavior can be severely affected, reproductive damage causes a decline in the number of brood size, and the speeding-up of the breeding rate is related to the concentration of TCS concentration-response. Key words: Triclosan; Caenorhabditis elegans; Locomotory behavior; Brood size; Generation time