The DUF288 domain containing proteins GhSTLs participate in cotton fiber cellulose synthesis and impact on fiber elongation

Abstract

Cotton is one of the most important economic crops in the world, with over 90 % cellulose in the mature fiber. However, the cellulose synthesis mechanism in cotton fibers is poorly understood. Here, we identified four DUF288 domain containing proteins, which we designated GhSTL1-4. These four GhSTL genes are highly expressed in 6 days post anthesis (dpa) and 20 dpa cotton fibers. They are localized to the Golgi apparatus, and can rescue the growth defects in primary cell wall (PCW) and secondary cell wall (SCW) of cellulose synthesis of the Arabidopsis stl1stl2 double mutant at varying degrees. Silencing of GhSTLs resulted in reduced cellulose content and shorter fibers. In addition, split-ubiquitin membrane yeast two-hybrid analysis showed that GhSTL1 and GhSTL4 can interact with PCW-related GhCesA6-1/6-3 and SCW-associated GhCesA7-1/7-2. GhSTL3 can interact with SCW-related GhCesA4-3. These interactions are further confirmed by firefly luciferase complementation imaging assay. Together, we demonstrate that GhSTLs can selectively interact with both the PCW and SCW-associated GhCesAs and impact on cellulose synthesis and fiber development. Our findings provide insights into the mechanism underlying cellulose biosynthesis in cotton fibers, and offer potential candidate genes to coordinate PCW and SCW cellulose synthesis of cotton fibers for developing elite cotton varieties with enhanced fiber quality.