Abstract
Methods PC12 cells and primary mesencephalic neurons were used in culture, and the striatum and the substantia nigra were prepared from wild-type and P2X7 receptor knockout mice. Rotenone and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatments were applied in vitro and in vivo to reproduce neurochemical hallmarks of PD. Receptor expression, cell survival indicators, and endogenous biogenic amine, amino acid, adenine nucleotide and endocannabinoid contents were analyzed.
Highlights
Previous studies indicate a role of P2X7 receptors in processes that lead to neuronal death
P2X7 antagonists protected against MPTP- and rotenoneinduced toxicity in the LDH assay, but failed to protect after rotenone treatment in the MTT assay in PC12 cells and in primary midbrain culture
In vivo MPTP and in vitro rotenone pretreatments increased the mRNA expression of P2X7 receptors in the striatum and substantia nigra of wild-type mice
Summary
The double-faced role of P2X7 receptors in toxininduced animal models of Parkinson’s disease. Joint meeting with the Hungarian Society of Experimental and Clinical Pharmacology (MFT) Innsbruck, Austria. 29-30 September 2011
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
