Abstract
Icariin had been reported as a potential agent for osteogenesis, but the dose-effect relationship needed further research to realize the clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) and stimulated them with different concentrations of icariin. The cytotoxicity of icariin was evaluated by the methylthiazolytetrazolium (MTT) assay method. The proliferation and osteogenic differentiation of such hBMSCs were investigated for different concentrations of icariin. We found that icariin had a dose-dependent effect on the proliferation and osteogenic differentiation of hBMSCs in a suitable concentration range from 10−9 M to 10−6 M, but at concentrations above 10−5 M, the cytotoxicity limited its use. The extremely low cost of icariin and its high abundance make it appealing for bone regeneration.
Highlights
The treatment of serious osseous defects remains a great challenge in orthopedic surgery [1]
Icariin showed positive effects on the proliferation and osteogenic differentiation in many recent studies, and the mechanism by which icariin enhances the proliferation and differentiation was mainly through bone morphogenetic proteins (BMPs)-2 and Cbfa1 expression [10,11,12], but its dose-effect relationship needed further research to realize the clinical application of icariin
The negative expression of hematopoietic markers in this study indicates no contamination by hematopoietic cells in the human bone mesenchymal stem cells (hBMSCs) isolation [13]
Summary
The treatment of serious osseous defects remains a great challenge in orthopedic surgery [1]. Numerous GFs, such as bone morphogenetic proteins (BMPs), platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), and insulin-like growth factors (IGFs), have been extensively studied and showed promisingly positive effects on the proliferation or/and differentiation of seed cells [4,5]. Epimedii herba showed a therapeutic effect on osteoporosis animal models [8,9]. Icariin showed positive effects on the proliferation and osteogenic differentiation in many recent studies, and the mechanism by which icariin enhances the proliferation and differentiation was mainly through BMP-2 and Cbfa expression [10,11,12], but its dose-effect relationship needed further research to realize the clinical application of icariin. The cytotoxicity, proliferation and osteogenic differentiation of such hBMSCs were investigated and compared
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