The DnaJ Gene Family in Pepper (Capsicum annuum L.): Comprehensive Identification, Characterization and Expression Profiles.

Fangfei Fan,Yuan Cheng,Xian Yang,Yunyan Kang,Xirong Chai

doi:10.3389/fpls.2017.00689

Fangfei Fan, Yuan Cheng + Show 3 more

Open Access

https://doi.org/10.3389/fpls.2017.00689

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Abstract

The DnaJ proteins which function as molecular chaperone played critical roles in plant growth and development and response to heat stress (HS) and also called heat shock protein 40 based on molecular weight. However, little was reported on this gene family in pepper. Recently, the release of the whole pepper genome provided an opportunity for identifying putative DnaJ homologous. In this study, a total of 76 putative pepper DnaJ genes (CaDnaJ01 to CaDnaJ76) were identified using bioinformatics methods and classified into five groups by the presence of the complete three domains (J-domain, zinc finger domain, and C-terminal domain). Chromosome mapping suggested that segmental duplication and tandem duplication were occurred in evolution. The multiple stress-related cis-elements were found in the promoter region of these CaDnaJ genes, which indicated that the CaDnaJs might be involved in the process of responding to complex stress conditions. In addition, expression profiles based on RNA-seq showed that the 47 CaDnaJs were expressed in at least one tissue tested. The result implied that they could be involved in the process of pepper growth and development. qRT-PCR analysis found that 80.60% (54/67) CaDnaJs were induced by HS, indicated that they could participated in pepper response to high temperature treatments. In conclusion, all these results would provide a comprehensive basis for further analyzing the function of CaDnaJ members and be also significant for elucidating the evolutionary relationship in pepper.

Highlights

With the increase of global warming, high temperature has become one of the most vital abiotic stresses on crop plants (Glazebrook, 1999)
A total of 85 putative sequences of pepper DnaJ genes were gotten from PGD by Hidden Markov Model (HMM) search
The CaDnaJs shared a conserved J-domain comprised about 70 amino acids, in which CaDnaJ57 owned the shortest J-domain with 39 amino acids, while J-domain of CaDnaJ25 was the longest (84 amino acids)

Summary

Introduction

With the increase of global warming, high temperature has become one of the most vital abiotic stresses on crop plants (Glazebrook, 1999). Pepper (Capsicum annuum L.) which originated in the tropical regions of Latin America had been widely cultivated around the world as an important vegetable crop nowadays and is sensitive to high temperature during plant growth and development, especially in reproductive stage (Guo et al, 2014). Previous researches had reported that heat shock response (HSR) was induced in many plant species under HS condition (Vierling, 1991). A great deal of ubiquitous and evolutionaryconserved proteins was identified as heat shock proteins (Hsps), one of the main products of the HSR (Vierling, 1991). According to approximate molecular weight and sequence homology, the Hsps were classified into five families, including the Hsp100, Hsp, Hsp, Hsp and small Hsps (Wang et al, 2004; Kotak et al, 2007; Gupta et al, 2010)

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