The dnaB gene product of Escherichia coli. II. Single stranded DNA-dependent ribonucleoside triphosphatase activity.

Abstract

The single-stranded DNA-dependent ribonucleoside triphosphatase activity of the Escherichia coli dnaB gene product was characterized. Purine ribonucleoside triphosphates were the preferred substrates, but all ribonucleoside triphosphates were cleaved at the gamma position to yield ribonucleoside diphosphates and Pi. The enzyme required Mg2+, which could be replaced by Mn2+ but with lower activity. The pH optimum was 7.5 in either Tris-HCl or phosphate buffer. The Km for MgATP was 0.59 mM and the Vmax was 8.7 nmol/min/microgram of protein at 30 degrees. The DNA requirement was best satisfied with either fd or phiX174 single-stranded DNA (Km 0.033 mM nucleotides); maximal rate of nucleoside diphosphate formation occurred with 1 dnaB molecule/fd or phiX174 single-stranded DNA molecule. The dnaB gene product was found to have hysteretic properties and the hysteresis appeared to be due to a dissociation and reassociation of the enzyme.