Abstract
The rates of DNA alkylation were established for the reaction of (+)-duocarmycin SA ( 1) with the native duplex d(G 1TCAATT A GTC 11) d(G 12ACTAATTGAC 22), an 11 bp deoxyoligonucleotide that contains a single high-affinity alkylation site that has been structurally characterized at exquisite resolution, and modified duplexes in which the four backbone phosphates proximal to the C4 carbonyl of bound 1 were replaced with methylphosphonates. All were found to react at comparable rates establishing that these backbone phosphates do not participate in catalysis of the DNA alkylation reaction.
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