Abstract
osteoblasts. During a 3-week culture of differentiating human osteoblast-like cells, TRPV6 and calbindin-D28K increased in the last week of culture, when mineralisation of the extracellular matrix takes place. Lentiviral overexpression of TRPV6 during osteoblasts differentiation caused a strongly elevated TRPV6 expression (>1000-fold) and most interestingly an almost 100% increase in mineralisation. Using microCT, we analysed femurs of 6-month old male wild type and TRPV6 knock-in mice. Despite similar bodyweights, femurs of TRPV6 knock-in mice were shorter (-4.2%). In addition, cortical and endocortical volume were significantly reduced compared to wild types (-11.3 and -14.7%, respectively). There were no significant alterations in trabecular and cortical thickness. In conclusion, the expression patterns of TRPV6 and other members of the transepithelial Ca transport machinery as well as the overexpression of TRPV6 in osteoblasts parallels mineralization and functional analyses indicate a positive role in mineralization. Loss of TRPV6 in vivo results in a bone phenotype but additional in-depth qBEI analyses will provide detailed mineralisation-related outcome following TRPV6 deficiency. Disclosure of Interest: None declared
Published Version
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