Abstract
In situ hybridization using 3H-RNA probes has been used to localize the sequences found in two satellites of density 1.705 g/cc and 1.672 g/cc to specific sites within the chromosomal complement. A detailed analysis of the sites on the S chromosome was carried out using the acute series of inversions to relate the heterochromatic breakpoint relative to the location of the sequence on this chromosome. It has also been possible to establish the order of arrangement of 1.705 and 1.672 DNA at the heterochromatic-euchromatic junction on chromosome 3(R). A mitotic map is provided. The Tm of hybrids formed in situ showed that the hybrids were representative of the sequences being analyzed. The two satellites also were traced through a number of purification procedures to show that a covalent linkage may be likely between the 1.705 g/cc and 1.672 g/cc satellite as predicted from in situ hybridization analyses.
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