Abstract

When centrifuged to equilibrium in neutral CsCl approximately 5% of the total nuclear DNA of V. melanops separated into a light satellite fraction. Buoyant density gradient analysis, thermal denaturation analysis and Cot reassociation experiments were used to find out the base sequence organisation of the satellite DNA. Using the method of in situ hybridisation its distribution in the chromosome complements of V. melanops and three other Vicia species were compared.

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