The distribution of ganglion cells in the posterior cricoarytenoid muscle of the normal adult rat. A light and electron microscopic study.

Abstract

We employed by light and electron microscopy to examine the innervation of the posterior cricoarytenoid muscle of the adult rat. The laryngeal nerve was found to often bifurcate into two different bundles. One contained large myelinated (motor) nerve fibers, which were located along the frontal (ventral) muscle surface and entered the muscle at its middle portion to form neuromuscular contacts with individual muscle fibers. The other nerve bundle consisting of clustered ganglion cells (20-30 microm in diameter) and their associated nonmyelinated and small-sized myelinated nerve fibers were mainly found on the dorsal side of the muscle and often ran along the peripheral clefts or depressions of the muscle surface. The nerve bundle often extended side branches, which entered the muscle to be distributed among muscle fibers and near arterioles. Some ganglion cells are considered to enter the muscle, accompanied by branched nerves. Intramuscular ganglion cells and their associated nerve fibers examined by electron microscopy were similar in fine structure to perimuscular ganglion cells and their associated nerve fibers. Nerve fibers contained abundant clear synaptic vesicles which were cholinergic in nature, and often formed synapses with both neighboring axons and the cell body of the ganglion cells. These findings suggest that, in the rat posterior cricoarytenoid muscle, perimuscular and intramuscular ganglion cells exist and may be involved in innervating and contracting smooth muscle cells of the arterioles, thus regulating the blood flow or intravascular pressure.