Abstract
The distribution is reported of alkaline phosphatase in the human tooth germ from the time of initiation up to and including the stage where the first increments of enamel matrix have formed. The early occurrence of enzyme activity in the stellate reticulum is considered to be associated with the determination of the crown pattern of the tooth. The gradient of enzyme activity in the components of the enamel organ is thought to reflect metabolic activity. Particular attention is drawn to the presence of cell layers rich in alkaline phosphatase, the sub-odontoblast layer and the stratum intermedium. The significance of this finding in relation to the production of calcified tissues is discussed. The absence of alkaline phosphatase activity in odontoblasts and ameloblasts is reported and discussed in relation to current views on hard tissue genesis.
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