Abstract
The regulation of the hepatic acetaldehyde (AcH) level during ethanol oxidation was investigated in vivo in fed male and female Sprague-Dawley rats. Various doses of ethanol were administered orally, the livers were freeze-clamped during pentobarbital anaesthesia, and ethanol, AcH, lactate, pyruvate, acetoacetate, 3-hydroxybutyrate and the aldehyde dehydrogenase activity were measured. A positive correlation was found between the ethanol and AcH concentration, when the ethanol concentrations were between 5–30 μmole/g wet wt liver. A negative correlation was found within this ethanol range between the hepatic mitochondrial free NADH/free NAD + ratio and the AcH concentration. A negative correlation was also obtained between the hepatic aldehyde dehydrogenase activity and the AcH concentration. A positive correlation was demonstrated between the hepatic mitochondrial free NADH/free NAD + ratio and the AcH concentration at ethanol concentrations above 30 μmole/g. The results are discussed in relation to the regulation of the hepatic AcH level by the metabolism of ethanol and AcH within the liver.
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