Abstract
In non-invasive prenatal screening (NIPS) via cell-free DNA (cfDNA), demise of a male co-twin may lead to discordant fetal sex prediction due to persistent chromosome Y contribution with a surviving female fetus. Despite this well-known limitation, the detection and clearance rate of chromosome Y cfDNA in vanished twin pregnancies has not been well described. Here chromosome Y contribution between serial NIPS samples is examined in 49 vanished twin pregnancies.
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